Document Details |
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| Name of Department/Committee : | FAD 31 |
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| Document Number : | FAD 31 ( 25884) |
| Document Title [English] : | Water Quality Detection and enumeration of Pseudomonas aeruginosa Part 2: Most probable number method |
| Document Title [Hindi] : | पानी की गुणवत्ता — स्यूडोमोनास एरुगिनोसा का पता लगाना और गणना करना — भाग 2: सबसे संभावित संख्या विधि |
| Document Type : | New |
| Language : | English |
| Priority : | 2 |
| ICS Code : | 13.060.70 |
| Date of Project Approval : | 28-04-2023 |
| Standards to be Superseded : | |
Classification Details |
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| Group : | Food, Food Products and food processing equipments |
| Sub Group : | Food safety |
| Sub Sub Group : | Detection of food borne pathogens |
| Aspects : | Methods of tests |
| Risk : | High |
| Certification : | Not Certifiable |
| Short Commom Man's Title : | Water Quality — Detection and enumeration of Pseudomonas aeruginosa — Part 2: Most probable number m |
| ITCHS Code : | |
| Ministry : |
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| Sustainable development Goals : |
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| Degree of Equivalence : | Identical under dual numbering |
| Identical/Equivalent Standards : | ISO 16266-2 : 2018 |
| Organization Type: | ISO |
| Sl.No. | Synosis Points | |
| 1 | This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the most probable number (MPN) of organisms by reference to MPN tables. | |
| 2 | This document is applicable to a range of types of water. For example, hospital waters, drinking water and non‐carbonated bottled waters intended for human consumption, groundwater, swimming pool and spa pool waters including those containing high background counts of heterotrophic bacteria. | |
| 3 | This document does not apply to carbonated bottled waters, flavoured bottle waters, cooling tower waters or marine waters, for which the method has not been validated. These waters are, therefore, outside the scope of this document. Laboratories can employ the method presented in this document for these matrices by undertaking appropriate validation of performance of this method prior to use. | |
| 4 | The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7‐amino‐4‐methylcoumarin aminopeptidase substrate present in a special reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7‐amido‐coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells. | |
| 5 | The standard also includes normative references, terms and definitions, principle, apparatus and glassware, culture media, diluents and reagents, sampling, preparation of test sample, procedure, expression of result, quality assurance and test report. | |
Timeline Details |
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Stages |
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| Sl. No. |
Stage | Date of Occurence | Remarks | Circulated to | Files |
| 1 | Generation of Document Number | 28-04-2023 | ------- | ||
| 2 | P-Draft Waived | 03-07-2024 | Since, draft standard is the adoption of an ISO standard developed by ISO TC 147 SC 4 where FAD 31 Sectional Committee (NMC of ISO TC147 SC 4) is participating actively, the P draft is being waived off. | ------- | |
| 3 | WC Draft | 03-07-2024 | Duration : 60 Days Submitted for HOD approval Remarks : May like to approve for wide circulation please |
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| 4 | WC Draft | 04-07-2024 | WC approved by HOD |
FADC | ------- |
| 5 | WC Draft | 04-07-2024 | WC approved by HOD |
FADC | ------- |